Question

Write a short note on Micro propagation.

Answer 1

Micro propagation: This is the propagation of plants involving cell and tissue culture. If vegetative propagation is not possible in a crop, then buds, shoot apex or any other part of the plant can be used as explant for micropropagation.

1. The explants are treated with sterilisation chemicals to prevent microbial growth, and then cultured in a particular nutrient medium.
2. Cells grow and divide to form a cell mass called callus Some growth regulators (Plant hormones) are added.
3. The callus differentiates into plant parts looking like plant (plantlet). After 4-6 weeks the plantlets transferred to the soil