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प्रश्न
Which one of the following is NOT true regarding restriction endonucleases?
They are ______.
पर्याय
used for cloning DNA in PCR
synthesized by bacteria as defense mechanism to destroy viral DNA
used in genetic engineering for ligation of DNA fragments
used to recognize short nucleotide sequence and cleave the DNA within them
MCQ
रिकाम्या जागा भरा
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उत्तर
They are used in genetic engineering for ligation of DNA fragments.
Explanation:
Restriction endonucleases (restriction enzymes) recognize short specific DNA sequences and cleave them; they are bacterial defence enzymes and are used to cut DNA for cloning. The joining (ligation) of DNA fragments is performed by DNA ligase, and PCR uses a thermostable DNA polymerase rather than restriction enzymes.
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