Question

What is recombinant DNA technology called popularly? Name the vectors and enzymes used in this technique.

Answer 1

Recombinant DNA technology is popularly called Genetic Engineering.

The main vectors used are:

• Plasmids: Small, circular DNA molecules used to carry foreign DNA into host cells.
• Bacteriophages (Phages): Viruses that infect bacteria and can carry foreign DNA.

Important enzymes used in this technology include:

• Restriction enzymes (Restriction endonucleases): Molecular scissors that cut DNA at specific sites to create fragments.
• DNA ligase: Enzyme that joins DNA fragments by forming phosphodiester bonds.