Question

The DNA molecules of the same size were extracted from E. coli and Plasmodium vivax. It was discovered that both the DNA molecules had one target site each for the restriction enzyme Hind II. After being digested with Hind II, the DNA fragments were subjected to gel electrophoresis.

With reference to the diagram given below, identify the lanes that represent the DNA fragments of E. coli and Plasmodium vivax respectively. Justify your answer with a reason for each.

Answer 1

Lane A – DNA of E. coli

Lane B – DNA of Plasmodium vivax

Because the circular DNA of E. coli was cut to release just one fragment, while the linear DNA of Plasmodium was cut to release two fragments.