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प्रश्न
Differentiate between the following:
Helicases and DNA ligase
फरक स्पष्ट करा
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उत्तर
| Basis of Difference | Helicases | DNA ligase |
|---|---|---|
| Main function | Helicases unwind and separate the two DNA strands. | DNA ligase joins separate sections of newly synthesised DNA. |
| Action on DNA | Helicases break the hydrogen bonds between complementary bases. | DNA ligase seals breaks in the sugar-phosphate backbone by forming phosphodiester bonds. |
| Role in replication | Helicases open the DNA double helix at the replication fork. | DNA ligase joins Okazaki fragments on the lagging strand. |
| Effect | Helicase activity exposes the DNA strands so they can act as templates. | Ligase activity makes a continuous DNA strand from separate DNA fragments. |
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