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प्रश्न
What are cry-proteins?
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उत्तर
Bacillus thuringiensis secretes harmful proteins known as insecticidal proteins in the form of cry proteins during a certain growth step. Cry, a gene, codes for the toxin. B. thuringiensis cry protein-encoding genes, often known as Bt toxin genes, have been identified and inserted into numerous crop plants, including Bt cotton, Bt corn, etc., to give insect pest resistance.
संबंधित प्रश्न
What is mycoherbicide?
How has the use of Agrobacterium as vectors helped in controlling Meloidegyne incognitia infestation in tobacco plants? Explain in correct sequence.
Cry-genes have been introduced in:
Silencing of a gene could be achieved through tee use ______.
Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produces (in the host cells).
In view of the current food crisis, it is said, that we need another green revolution. Highlight the major limitations of the earlier green revolution.
Gene expression can be controlled with the help of RNA. Explain the method with an example.
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RNA interference (RNAi) holds great potential as a therapeutic agent for the treatment of human diseases and as biocontrol agents in controlling pests in the field agriculture. An experiment was carried to study the use of 'RNAi' for the potential treatment of disorders of cholesterol metabolism. Some people possess genetic mutations with elevated levels of ApoB gene which predisposes them to coronary artery diseases. Lowering the amount ApoB can reduce the number of lipoproteins and lower the blood cholesterol. Tracy Zimmerman and her colleagues used RNAi in 2006 to reduce the level of ApoB in non human primates Cynomolgus monkeys. One group of monkeys were given RNAi treatment (small interfering RNAs, SiRNAs) (doses 1 mg/kg, SiRNAs), second group of monkeys were given RNAi treatment (doses 2.5 mg/kg, SiRNAs) and third group of monkeys were injected with saline. |
The results of the study are depicted in the graph below:
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- Write any two natural sources from where dsRNA molecule could be obtained for silencing the specific mRNA.
- How is RNAi used in controlling the infection on the roots of tobacco plants by the nematode Meloidogyne incognitia?
Certain specific bacterial spores are mixed in water and sprayed over Brassica crop to control butterfly caterpillars.
Name this bacterium and its mode of action on the butterfly caterpillars.
"RNA interference has been used to produce transgenic tobacco plants to protect them from the infestation by specific nematodes." Explain the novel strategy exploited by the biotechnologists.
