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NCERT solutions Biology Textbook for Class 12 chapter 11 Biotechnology : Principles and Processes

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Chapter 11 - Biotechnology : Principles and Processes

Pages 205 - 206

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).

Q 1 | Page 205 | view solution

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Q 2 | Page 205 | view solution

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

Q 3 | Page 205 | view solution

What would be the molar concentration of human DNA in a human cell? Consult your teacher.

Q 4 | Page 205 | view solution

Do eukaryotic cells have restriction endonucleases? Justify your answer.

Q 5 | Page 205 | view solution

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?

Q 6 | Page 205 | view solution

Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.

Q 7 | Page 205 | view solution

Can you recall meiosis and indicate at what stage a recombinant DNA is made?

Q 8 | Page 205 | view solution

Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?

Q 9 | Page 205 | view solution

Describe briefly the following:

Origin of replication

 

Q 10.1 | Page 206 | view solution

Describe briefly the following

Bioreactors

Q 10.2 | Page 206 | view solution

Describe briefly the followings:

Downstream processing

Q 10.3 | Page 206 | view solution

Explain briefly - PCR: - Polymerase chain reaction (PCR)

Q 11.1 | Page 206 | view solution

Explain briefly Restriction enzymes and DNA

Q 11.2 | Page 206 | view solution

Explain briefly Chitinase

Q 11.3 | Page 206 | view solution

distinguish between Plasmid DNA and chromosomal DNA

Q 12.1 | Page 206 | view solution

distinguish between RNA and DNA

Q 12.2 | Page 206 | view solution

distinguish between Exonuclease and Endonuclease

Q 12.3 | Page 206 | view solution

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