BE Biotechnology Semester 8 (BE Fourth Year)University of Mumbai

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Protein Engineering Semester 8 (BE Fourth Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2019 - current]
CBGS [2015 - 2018]
Old [2000 - 2014]

Topics with syllabus and resources

100.00 Structure of Proteins
  • Post translational Modifications of proteins.
  • Primary Structure and its determination
  • Ramchandran Plot
  • Secondary, Tertiary and Quaternary Structure of Proteins
  • Bonds that stabilize a protein molecule
  • Protein folding pathways and Energy Status of a Protein Molecule
  • Protein Degradation in the cell
200.00 Techniques Involved in Studying Protein Structure
  • Methods of protein crystallization.
  • Methods to study the quaternary structures of proteins: X-ray Crystallography, NMR Spectroscopy.
300.00 Structure Function Relationships in Proteins
  • Helix-turn-Helix motif in DNA binding and homeodomain protein.
  • Zinc fingers.
  • Leucine zippers.
  • Membrane proteins: General characteristics, Transmembrane segments, bacteriorhodopsin and Photosynthetic reaction center
400.00 Concepts of Designing a New Protein Molecule
  • Chemical synthesis of peptides.
  • Target molecules for Protein Engineering.
  • The protein cycle and steps involved in Engineering a new Protein.
  • De novo protein design
500.00 Applications of Protein Engineering
  • Protein Engineering to enhance the solubility and assist folding of expressed proteins.
  • Protein Engineering to assist purification of expressed proteins.
  • Role in Vaccine Development.
  • Engineering blood clotting factors: factor VIII.
  • Engineering enzymes: tyrosyl-tRNA synthase.
  • Engineering therapuetic hormones: insulin.
  • Engineering humanized antibodies
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