BE Biotechnology Semester 4 (SE Second Year)University of Mumbai

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Topics and Syllabus - BE Biotechnology Molecular Genetics Semester 4 (SE Second Year) University of Mumbai

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CBCGS [2017 - current]
CBGS [2013 - 2016]
Old [2000 - 2012]

Topics with syllabus and resources

1 Structure of Nucleic Acid
  • DNA, RNA: mRNA,tRNA, rRNA,
  • Denaturation and Renaturation of DNA,
  • Tm; GC content from Tm,
  • Renaturation kinetics of DNA
  • Complexity of DNA, Cot curves
  • Satellite DNA: Repetitive DNA, SNP, STR
2 Mendelism and Its Extensions
  • Law of segregation
  • Law of independent assortment
  • Chromosomal basis of segregation and independent
  • Assortment
  • Linkage
  • Crossing over
  • Multiple allelism
  • Pleiotropy
  • Recombination
3 Cytogenetics
  • International System for Human Chromosome Nomenclature
  • Mechanisms of numerical and structural chromosomal aberrations
  • Chromosomal basis of sex determination
  • Non-chromosomal basis of sex determination; mutations
4 DNA Replication
  • Prokaryotic and eukaryotic DNA replication,
  • Mechanism of DNA replication
  • Enzymes and accessory proteins involved in DNA replication.
  • DNA Damage Mechanism
  • DNA Repair Mechanism
5 Transcription
  • Prokaryotic transcription
  • Eukaryotic transcription
  • RNA polymerases,
  • General and specific transcription factors
  • Regulatory elements and mechanisms of transcription regulation, 5'-Cap formation
  • Transcription termination
  • 3'-end processing and polyadenylation, Post-transcriptional gene silencing RNA splicing
  • Nuclear splicing: splice some
  • Group I and group II introns
  • tRNA splicing
  • Alternate splicing
6 Translation
  • Prokaryotic and eukaryotic translation
  • Synthesis of aminoacyl tRNA
  • Aminoacylsynthetases
  • Mechanism of initiation, elongation and termination
  • Regulation of translation, co-and post-translational modifications of proteins
7 Regulation of Gene Expression
  • Induction and repression,
  • Operon theory, lac operon, trp operon, ara operon
  • Attenuation
  • Positive and Negative Control
  • Catabolite repression
  • Regulation of transcription by cAMP and CRP
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