BE Biotechnology Semester 3 (SE Second Year)University of Mumbai
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Microbiology Semester 3 (SE Second Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2017 - current]
CBGS [2013 - 2016]
Old [2000 - 2012]

Topics with syllabus and resources

100.00 History and Scope of Industrial Microbiology
  • Introduction: Discovery of Microbial world
  • The experiments of Pasteur; The discovery of Anaerobic Life
  • Physiological significance of Fermentation; Pasteur and Fermentation
  • The Era of discovery of Antibiotics; Growth of Industrial fermentation
200.00 Classification of Micro Organisms

1. Types and general characteristics of microorganisms:-

  • BacteriaArchaebacteria, Actinomycetes, Rickettsia, Mycoplasma, Chlamydia
  • Fungi – Molds and yeasts
  • Algae
  • Protozoa
  • Viruses

2. The classification of bacteria Species:-

  • The unit of classification,
  • New approaches to bacterial taxonomy,
  • Bacterial taxonomy the problems of taxonomic arrangements,
  • Bacterial phylogeny.
  • Aerobic and Anaerobic cultures
300.00 Microbial Pathogenesis
  • Epidemiology of infectious diseases, Bacterial, Fungal, Protozoal, Viral Diseases;
  • Bacterial invasion and colonization
  • Bacterial toxins- types and mode of action
400.00 Microbial Nutrition
  • Nutritional requirements of microorganisms
  • Different types of media- Synthetic media, complex media Selective media, differential media, enrichment media.
500.00 Microbial Growth
  • Introduction: phases of growth
  • Growth curve
  • Kinetics of growth
  • Measurement of growth
  • Continuous & batch culture
  • Synchrony
  • Chemostat & turbidostat.
  • Effects of solutes ,temperature ,ion concentration, oxygen, hydrostatic pressure ,heavy metal ions, and UV light on microbial growth
600.00 Microbiological Techniques
  • Sterilization and disinfection techniques,
  • Principles and methods of sterilization.
  • Physical methods - autoclave, hot-air oven, pressure cooker, laminar air flow, filter sterilization.
  • Radiation methods – UV rays, gamma rays, ultrasonic methods.
  • Chemical methods - Use of alcohols, aldehydes, fumigants, phenols, halogens and hypochlorites.
  • Phenol coefficient.
  • Isolation of pure culture techniques - Enrichment culturing, dilutionplating, streak- plate, spread-plate and micromanipulator.
  • Preservation of microbial cultures - sub culturing, overlaying cultures with mineral oils, lyophilization, sand cultures, storage at low temperature.
700.00 Antimicrobial Therapy
  • Antimicrobial sensitivity tests.
  • Agents used in treating infection:- Antibacterial, antiviral, anti retroviral, antifungal, anti-protozoan & anti helminthes.
  • Resistance mechanism
800.00 Water and Soil Microbiology

1. Microbiological analysis of water purity:-

  • sanitary tests for coliforms (presumptive test, confirmed test, competed test),
  • MPN test,
  • defined substrate test,
  • IMVIC test.

2.Soil microbiology:-

  • soil as a habitat for microorganisms,
  • physico chemical properties of soil,
  • microbial community in soil,
  • role of microorganisms in organic matter decomposition
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