BE Biotechnology Semester 5 (TE Third Year)University of Mumbai

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Genetic Engineering Semester 5 (TE Third Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2018 - current]
CBGS [2014 - 2017]
Old [2000 - 2013]

Topics with syllabus and resources

100.00 DNA
  • Structure
  • Topology
  • Superhelical and relaxed molecules.
  • Plasmids in nature
  • Fundamentals of Density gradient Centrifugation and Alkaline lysis for Plasmid preparation.
200.00 Palindromes and Repeat Structures
  • Restriction Endonucleases and their action.
  • Vectors used for Gene-cloning:-
  • Plasmids (e.g pUC type, conjugative, Ti etc.)
  • Phages (Lambda and M13 type)
  • Cosmids and Phagemids.
300.00 Cloning Strategies
  • Expression and Fusion vectors Stability of plasmids(PAR locus, High copy number etc)
  • Library construction (Genomic and C-DNA type)
  • Screening for the clone.
400.00 DNA Transfer into Cells
  • Transformation and Transfection
  • Membrane Fusion and Electroporation
  • Gene-Gun and Micro-injection
500.00 Enzymes for Recombinant Technology
  • Restriction Endonucleases
  • DNA polymerases
  • Reverse Transcriptase
  • Polynucleotide Kinase
  • Terminal Transferase
  • Alkaline Phosphatase
  • S1-Nuclease
  • Bal-31
  • DNA Ligase
600.00 DNA and Protein Analysis
  • DNA: Southern and Northern Hybridization.
  • PCR Amplification
  • DNA Sequence Analysis (e.g Sangers Method)
  • Automated Sequencing
  • RFLP and RAPD.
  • Protein: Western Blotting, ELISA and its variations.
700.00 Antisense and Rna Interference Technology and Their Applications
  • r-DNA in medicine
  • e.g. Insulin and Blood clotting factor VIII.
  • Use of cell-lines in bio-molecules production.
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