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Genetic Engineering Semester 5 (TE Third Year) BE Biotechnology University of Mumbai Topics and Syllabus

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University of Mumbai Syllabus For Semester 5 (TE Third Year) Genetic Engineering: Knowing the Syllabus is very important for the students of Semester 5 (TE Third Year). Shaalaa has also provided a list of topics that every student needs to understand.

The University of Mumbai Semester 5 (TE Third Year) Genetic Engineering syllabus for the academic year 2021-2022 is based on the Board's guidelines. Students should read the Semester 5 (TE Third Year) Genetic Engineering Syllabus to learn about the subject's subjects and subtopics.

Students will discover the unit names, chapters under each unit, and subtopics under each chapter in the University of Mumbai Semester 5 (TE Third Year) Genetic Engineering Syllabus pdf 2021-2022. They will also receive a complete practical syllabus for Semester 5 (TE Third Year) Genetic Engineering in addition to this.

CBCGS [2018 - current]
CBGS [2014 - 2017]
Old [2000 - 2013]

University of Mumbai Semester 5 (TE Third Year) Genetic Engineering Revised Syllabus

University of Mumbai Semester 5 (TE Third Year) Genetic Engineering and their Unit wise marks distribution

University of Mumbai Semester 5 (TE Third Year) Genetic Engineering Course Structure 2021-2022 With Marking Scheme

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Syllabus

100 DNA
  • Structure
  • Topology
  • Superhelical and relaxed molecules.
  • Plasmids in nature
  • Fundamentals of Density gradient Centrifugation and Alkaline lysis for Plasmid preparation.
200 Palindromes and Repeat Structures
  • Restriction Endonucleases and their action.
  • Vectors used for Gene-cloning:-
  • Plasmids (e.g pUC type, conjugative, Ti etc.)
  • Phages (Lambda and M13 type)
  • Cosmids and Phagemids.
300 Cloning Strategies
  • Expression and Fusion vectors Stability of plasmids(PAR locus, High copy number etc)
  • Library construction (Genomic and C-DNA type)
  • Screening for the clone.
400 DNA Transfer into Cells
  • Transformation and Transfection
  • Membrane Fusion and Electroporation
  • Gene-Gun and Micro-injection
500 Enzymes for Recombinant Technology
  • Restriction Endonucleases
  • DNA polymerases
  • Reverse Transcriptase
  • Polynucleotide Kinase
  • Terminal Transferase
  • Alkaline Phosphatase
  • S1-Nuclease
  • Bal-31
  • DNA Ligase
600 DNA and Protein Analysis
  • DNA: Southern and Northern Hybridization.
  • PCR Amplification
  • DNA Sequence Analysis (e.g Sangers Method)
  • Automated Sequencing
  • RFLP and RAPD.
  • Protein: Western Blotting, ELISA and its variations.
700 Antisense and Rna Interference Technology and Their Applications
  • r-DNA in medicine
  • e.g. Insulin and Blood clotting factor VIII.
  • Use of cell-lines in bio-molecules production.
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