University of Mumbai Syllabus For Semester 5 (TE Third Year) Genetic Engineering: Knowing the Syllabus is very important for the students of Semester 5 (TE Third Year). Shaalaa has also provided a list of topics that every student needs to understand.
The University of Mumbai Semester 5 (TE Third Year) Genetic Engineering syllabus for the academic year 2022-2023 is based on the Board's guidelines. Students should read the Semester 5 (TE Third Year) Genetic Engineering Syllabus to learn about the subject's subjects and subtopics.
Students will discover the unit names, chapters under each unit, and subtopics under each chapter in the University of Mumbai Semester 5 (TE Third Year) Genetic Engineering Syllabus pdf 2022-2023. They will also receive a complete practical syllabus for Semester 5 (TE Third Year) Genetic Engineering in addition to this.
University of Mumbai Semester 5 (TE Third Year) Genetic Engineering Revised Syllabus
University of Mumbai Semester 5 (TE Third Year) Genetic Engineering and their Unit wise marks distribution
University of Mumbai Semester 5 (TE Third Year) Genetic Engineering Course Structure 2022-2023 With Marking Scheme
| # | Unit/Topic | Weightage |
|---|---|---|
| 100 | DNA | |
| 200 | Palindromes and Repeat Structures | |
| 300 | Cloning Strategies | |
| 400 | DNA Transfer into Cells | |
| 500 | Enzymes for Recombinant Technology | |
| 600 | DNA and Protein Analysis | |
| 700 | Antisense and Rna Interference Technology and Their Applications | |
| Total | - |
Syllabus
- Structure
- Topology
- Superhelical and relaxed molecules.
- Plasmids in nature
- Fundamentals of Density gradient Centrifugation and Alkaline lysis for Plasmid preparation.
- Restriction Endonucleases and their action.
- Vectors used for Gene-cloning:-
- Plasmids (e.g pUC type, conjugative, Ti etc.)
- Phages (Lambda and M13 type)
- Cosmids and Phagemids.
- Expression and Fusion vectors Stability of plasmids(PAR locus, High copy number etc)
- Library construction (Genomic and C-DNA type)
- Screening for the clone.
- Transformation and Transfection
- Membrane Fusion and Electroporation
- Gene-Gun and Micro-injection
- Restriction Endonucleases
- DNA polymerases
- Reverse Transcriptase
- Polynucleotide Kinase
- Terminal Transferase
- Alkaline Phosphatase
- S1-Nuclease
- Bal-31
- DNA Ligase
- DNA: Southern and Northern Hybridization.
- PCR Amplification
- DNA Sequence Analysis (e.g Sangers Method)
- Automated Sequencing
- RFLP and RAPD.
- Protein: Western Blotting, ELISA and its variations.
- r-DNA in medicine
- e.g. Insulin and Blood clotting factor VIII.
- Use of cell-lines in bio-molecules production.
