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Enzyme Engineering Semester 6 (TE Third Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2018 - current]
CBGS [2014 - 2017]
Old [2000 - 2013]

Topics with syllabus and resources

100.00 Enzyme as a Biological Catalysts
  • Chemical nature
  • polypeptide structures Models of Enzyme-substrate interactions
  • Catalytic and Allosteric sites
  • Activation Energy and catalysis
200.00 Structures of Enzymes
  • Structures of Enzymes (Primary, Secondary, Tertiary etc.)
  • Effect of pH
  • Temperature and Salts on Enzyme efficiency and Inhibition
  • Enzyme Kinetics
  • models and degree of efficiencies/Inhibition
  • their types and analysis
300.00 Enzyme Activity Analysis
  • pH-change
  • Viscometry
  • Manometry
  • Colorimetry
  • Spectrophotometry, etc.
  • Fundamentals of each method
  • constants and variables involved
  • Criteria for the Degree of accuracy.
400.00 Working with Enzyme
  • Detection
  • Isolation and Purification of Enzyme under study
  • Stability of Enzyme
  • Genetic Modification of Enzyme
  • Immobilization of Enzyme for repeat use
500.00 Criteria of Enzyme Purity, Specific Activity, Subunit Analysis, Western Blotting
600.00 Process Design and Operation Strategies for Enzyme Based Reactors
  • Operational problems
  • Decline and loss of Enzyme efficiency
  • Remedies
700.00 Application of Enzyme in Industries
  • Food
  • Leather
  • Beverage
  • Detergents
  • Pharmaceuticals and Medicines
  • Analytical/ Diagnostics and Biosensors
  • Therapeutics
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