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University of Mumbai Semester 6 (TE Third Year) Enzyme Engineering Revised Syllabus
University of Mumbai Semester 6 (TE Third Year) Enzyme Engineering and their Unit wise marks distribution
Units and Topics
| # | Unit/Topic | Marks |
|---|---|---|
| 100 | Enzyme as a Biological Catalysts | - |
| 200 | Structures of Enzymes | - |
| 300 | Enzyme Activity Analysis | - |
| 400 | Working with Enzyme | - |
| 500 | Criteria of Enzyme Purity, Specific Activity, Subunit Analysis, Western Blotting | - |
| 600 | Process Design and Operation Strategies for Enzyme Based Reactors | - |
| 700 | Application of Enzyme in Industries | - |
| Total | - |
Syllabus
100 Enzyme as a Biological Catalysts
- Chemical nature
- polypeptide structures Models of Enzyme-substrate interactions
- Catalytic and Allosteric sites
- Activation Energy and catalysis
200 Structures of Enzymes
- Structures of Enzymes (Primary, Secondary, Tertiary etc.)
- Effect of pH
- Temperature and Salts on Enzyme efficiency and Inhibition
- Enzyme Kinetics
- models and degree of efficiencies/Inhibition
- their types and analysis
300 Enzyme Activity Analysis
- pH-change
- Viscometry
- Manometry
- Colorimetry
- Spectrophotometry, etc.
- Fundamentals of each method
- constants and variables involved
- Criteria for the Degree of accuracy.
400 Working with Enzyme
- Detection
- Isolation and Purification of Enzyme under study
- Stability of Enzyme
- Genetic Modification of Enzyme
- Immobilization of Enzyme for repeat use
500 Criteria of Enzyme Purity, Specific Activity, Subunit Analysis, Western Blotting
600 Process Design and Operation Strategies for Enzyme Based Reactors
- Operational problems
- Decline and loss of Enzyme efficiency
- Remedies
700 Application of Enzyme in Industries
- Food
- Leather
- Beverage
- Detergents
- Pharmaceuticals and Medicines
- Analytical/ Diagnostics and Biosensors
- Therapeutics
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