BE Biotechnology Semester 6 (TE Third Year)University of Mumbai
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Cell and Tissue Culture Semester 6 (TE Third Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2018 - current]
CBGS [2014 - 2017]
Old [2000 - 2013]

Topics with syllabus and resources

100.00 Plant Tissue Culture Introduction
  • Internal organization of plant
  • Plant Tissue Culture Media
  • Plant growth hormones
  • Concept of Totipotency
  • Study of various types of Organ Culture
  • Organogenesis
  • Micropropagation
200.00 Plant Cell Culture and Its Applications
  • Plant Cell Suspension Culture
  • Single Cell Culture
  • Somatic Embryogenesis
  • Artificial Seeds
  • Protoplast Culture & Somatic Hybridization
  • Scale-up and Automation of Plant Cell Culture
300.00 Transformation of Plants
  • Agrobacterium mediated Gene transfer
  • Direct Methods of Gene Transfer
  • Chemical Methods
  • Electroporation
  • Microinjection
  • Particle Bombardment
  • Virus Mediated Gene Transfer
  • Transgenic Plants
  • Applications of Plant Biotechnology for Production of Quality Oil
  • Industrial Enzymes and Plantibodies
400.00 Introduction to Animal Cell Culture
  • Historical Background
  • Advantages of Tissue Culture
  • Limitations
  • Major Types of Tissue Culture
500.00 Laboratory
  • Laboratory Design & Layout of ATC laboratory
  • Equipments and Materials Of a Tissue Culture Laboratory
  • Media Preparation and Sterilization techniques
  • The Culture Environment
  • Cell Adhesion
  • Cell Proliferation
  • Differentiation
  • Cell Signaling
  • Energy Metabolism
600.00 Primary Culture
  • Initiation of a Primary Cell Culture
  • Isolation of the Tissue
  • Types of Primary Culture
  • Subculture and Cell Lines
700.00 Cloning and Selection of Animal Cells
  • Cell Separation
  • Characterization
  • Differentiation
  • Cryopreservation
  • Scale-up & Automation
  • Antibody Engineering and Large scale Production of Pharmaceutical Products
  • Stem cell Cultures
  • Embryonic Stem Cell Cultures and their Applications
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