BE Biotechnology Semester 8 (BE Fourth Year)University of Mumbai
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Bioseparation and Downstream Processing Technology 2 Semester 8 (BE Fourth Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2019 - current]
CBGS [2015 - 2018]
Old [2000 - 2014]

Topics with syllabus and resources

100.00 Adsorption and Ion Exchange
  • Introduction to Adsorption
  • Types of Adsorption:- Adsorption Isotherms, Single Stage Adsorption, Multistage Cross Current Adsorption, Multistage Counter Current Adsorption
  • Equipments for Adsorption Ion Exchange Equilibria, Ion Exchange Equipments Design and Construction of Chromatographic Columns for Bioseparations
200.00 Membrane Separation Techniques
  • Membrane separation processes: Reverse Osmosis, Ultrafiltration, Microfiltration, Nanofiltration, Dialysis, Electrodialysis, Gas Permeation, Pervaporation
  • Types of Membranes, Membrane Modules and design Retention coefficient, Concentration Polarization, Membrane fouling
  • Factors affecting membrane filtration
  • Advantages of membrane separation processes over conventional separation techniques
  • Industrial Applications
300.00 Equipments for Gas-liquid Contacting Applicable for Bioprocesses
  • Classification of equipments for gas-liquid contacting, Gas dispersed and liquid continuous phase- Sparged Vessels (Bubble Columns), Mechanically Agitated Vessels, Tray Towers, Spray Towers and Spray Chambers, Packed Towers
  • Comparison of Packed Towers with Tray Towers.
400.00 Crystallization
  • Solubility curve, Super saturation, Method of obtaining super saturation
  • Effect of heat on size and growth of crystal, Rate of Crystal growth and Delta-L law of crystal growth, Material and energy balance for crystallizers
  • Crystallization equipment-description
500.00 Drying
  • Introduction to drying, Equilibrium, Different types of moisture contents, Rate of Drying and drying curve, Batch Drying and calculation of time of drying, types of driers
  • Lyophilization
  • Formulation
600.00 Case Studies of Downstream Processing
  • Baker’s yeast, Ethanol, Citric acid, Penicillin, Insulin, interferon, Monoclonal antibodies, Tissue plasminogen activator, Taq polymerase
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