BE Biotechnology Semester 7 (BE Fourth Year)University of Mumbai
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Bioseparation and Downstream Processing Technology 1 Semester 7 (BE Fourth Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2019 - current]
CBGS [2015 - 2018]
Old [2000 - 2014]

Topics with syllabus and resources

100.00 Introduction to Bioproducts and Bioseparation
  • Range and characteristics of bioproducts
  • Characteristics of Fermentation Broth
  • Selection of unit operation with due consideration of physical, chemical and biochemical aspect of biomolecules
  • Stages of Downstream Processing
200.00 Product Release and Recovery Processes
  • Fundamental principles of obtaining the product from cell cultures: intracellular vs. extracellular product
  • Cell disruption-Physical, Chemical and Enzymatic methods of cell disruption
  • Mechanical Cell disruption methods: High pressure Cell Homogenizer, Bead Mill, Sonication
300.00 Primary Separation
  • Removal of insolubles and Biomass (and particulate debris) separation techniques
  • Flocculation and sedimentation
  • Centrifugation-Ultracentrifugation, Gradient centrifugation
  • Filtration: Theory of Filtration, Pretreatment of Fermentation Broth, Filter Media and Equipment, Conventional and Cross- flow Filtration, Continuous Filtration, Filter cake resistance, specific cake resistance, Washing and dewatering of filter cakes
400.00 Gas Absorption
  • Solubility of gases in liquids, Effect of temperature and pressure on solubility, Ideal and Non-ideal solutions, Choice of solvent for gas absorption, absorption factor,stripping factor, minimum gas liq ratio, Single stage gas absorption- Cross Current, Cocurrent, Countercurrent, Multistage Counter current Operation.
  • Absorption with Chemical Reactions Related problems
500.00 Liquid-liquid Extraction
  • Introduction to Liquid-Liquid Extraction, Choice of Solvent for Liquid-Liquid Extraction
  • Triangular coordinate system, Ternary Equilibria [Binodal Solubility Curve with effect of temperature and pressure on it], Single Stage Operation, Multistage Cross Current Operation, Multistage Counter Current Operation [with and without re- flux]
  • Equipments for liquid-liquid extraction.
  • Kinetics and modeling of extraction cycles
  • Types of extraction processes:- Reactive extraction, Aqueous two phase systems, Reverse micellar extraction, Liquid-liquid and solid-liquid extraction, Supercritical fluid Extraction.
  • Design of extraction equipment.
  • Different types of extractors and designing of extractors.
  • Leaching:- Representation of equilibria, single stage leaching, multistage cross current leaching, multistage counter current leaching, equipments for leaching.
600.00 Precipitation
  • Protein Precipitation methods: Isoelectric precipitation, Salting out, Organic solvent addition, Non-ionic polymers, Polyelectrolyte Addition
  • Selective denaturation of unwanted proteins
  • Large scale precipitation
  • Applications
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