BE Biotechnology Semester 4 (SE Second Year)University of Mumbai

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Analytical Methods in Biotechnology Semester 4 (SE Second Year) BE Biotechnology University of Mumbai Topics and Syllabus

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CBCGS [2017 - current]
CBGS [2013 - 2016]
Old [2000 - 2012]

Topics with syllabus and resources

100.00 Centrifugation
  • General principle- sedimentation velocity, sedimentation equilibrium
  • Types of centrifuges, preparative and analytical centrifugation, differential centrifugation, density gradient methods
  • Applications
200.00 Chromatographic Techniques
  • Introduction to chromatography, General principles
  • Planar Chromatography: Thin layer chromatography, paper chromatography
  • Column chromatography–columns, stationary phases. Packing of columns, application of sample, column development, fraction collection and analysis.
  • Partition chromatography, Adsorption chromatography Affinity Chromatography, Ion Exchange Chromatography, Chromatofocussing, Size exclusion chromatography.
  • Gas Chromatography, HPLC: Principle & Components: pumping systems, detectors systems
  • Applications
300.00 Electrokinetic Methods of Separation
  • Electrophoresis: General principle, factors affecting electrophoresis – voltage, current, resistance, buffer, composition, concentration, pH.
  • Agarose Gel electrophoresis
  • SDS-PAGE - gradient gels
  • Two dimensional gel electrophoresis
  • Isoelectric focusing
  • Capillary electrophoresis
  • Immunoelectrophoresis
400.00 Spectroscopy
  • Spectroscopic Techniques; Beers Lamberts law, molar and extinction coefficient, limitations of Beers Lamberts law.
  • Visible and UV Spectrophotometry; Principles, Instrumentation and applications
500.00 Radioisotopic Techniques
  • Use of radioisotopes in life sciences, radioactive labeling, principle and application of tracer techniques
  • Detection and measurement of radioactivity using ionization chamber, proportional chamber, Geiger-Muller and Scintillation counters, Autoradiography
  • Applications
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