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Analytical Methods in Biotechnology Semester 4 (SE Second Year) BE Biotechnology University of Mumbai Topics and Syllabus

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University of Mumbai Syllabus For Semester 4 (SE Second Year) Analytical Methods in Biotechnology: Knowing the Syllabus is very important for the students of Semester 4 (SE Second Year). Shaalaa has also provided a list of topics that every student needs to understand.

The University of Mumbai Semester 4 (SE Second Year) Analytical Methods in Biotechnology syllabus for the academic year 2021-2022 is based on the Board's guidelines. Students should read the Semester 4 (SE Second Year) Analytical Methods in Biotechnology Syllabus to learn about the subject's subjects and subtopics.

Students will discover the unit names, chapters under each unit, and subtopics under each chapter in the University of Mumbai Semester 4 (SE Second Year) Analytical Methods in Biotechnology Syllabus pdf 2021-2022. They will also receive a complete practical syllabus for Semester 4 (SE Second Year) Analytical Methods in Biotechnology in addition to this.

CBCGS [2017 - current]
CBGS [2013 - 2016]
Old [2000 - 2012]

University of Mumbai Semester 4 (SE Second Year) Analytical Methods in Biotechnology Revised Syllabus

University of Mumbai Semester 4 (SE Second Year) Analytical Methods in Biotechnology and their Unit wise marks distribution

University of Mumbai Semester 4 (SE Second Year) Analytical Methods in Biotechnology Course Structure 2021-2022 With Marking Scheme

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Syllabus

100 Centrifugation
  • General principle- sedimentation velocity, sedimentation equilibrium
  • Types of centrifuges, preparative and analytical centrifugation, differential centrifugation, density gradient methods
  • Applications
200 Chromatographic Techniques
  • Introduction to chromatography, General principles
  • Planar Chromatography: Thin layer chromatography, paper chromatography
  • Column chromatography–columns, stationary phases. Packing of columns, application of sample, column development, fraction collection and analysis.
  • Partition chromatography, Adsorption chromatography Affinity Chromatography, Ion Exchange Chromatography, Chromatofocussing, Size exclusion chromatography.
  • Gas Chromatography, HPLC: Principle & Components: pumping systems, detectors systems
  • Applications
300 Electrokinetic Methods of Separation
  • Electrophoresis: General principle, factors affecting electrophoresis – voltage, current, resistance, buffer, composition, concentration, pH.
  • Agarose Gel electrophoresis
  • SDS-PAGE - gradient gels
  • Two dimensional gel electrophoresis
  • Isoelectric focusing
  • Capillary electrophoresis
  • Immunoelectrophoresis
400 Spectroscopy
  • Spectroscopic Techniques; Beers Lamberts law, molar and extinction coefficient, limitations of Beers Lamberts law.
  • Visible and UV Spectrophotometry; Principles, Instrumentation and applications
500 Radioisotopic Techniques
  • Use of radioisotopes in life sciences, radioactive labeling, principle and application of tracer techniques
  • Detection and measurement of radioactivity using ionization chamber, proportional chamber, Geiger-Muller and Scintillation counters, Autoradiography
  • Applications
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