PUC Karnataka Science Class 12Department of Pre-University Education, Karnataka
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Write the steps you would suggest to be undertaken to obtain a foreign-gene-product. - PUC Karnataka Science Class 12 - Biology

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Question

Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.

Solution

Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps-
1. Identification and isolation of gene of interest
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule
3. Transfer of this recombinant DNA molecule into suitable host cell
4. Selection of recombinants and non-recombinant cells
5. Expression of gene of interest by culturing recombinant cells under suitable conditions
6. Culturing of cells in bioreactors for large scale production of desired product

  Is there an error in this question or solution?
Solution Write the steps you would suggest to be undertaken to obtain a foreign-gene-product. Concept: Tools of Recombinant Dna Technology - Competent Host (For Transformation with Recombinant Dna).
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