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With the Help of a Neat and Labelled Diagram Describe the Steps in Recombinant Dna Technology. - Plasmids

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With the help of a neat and labelled diagram describe the steps in recombinant DNA technology.

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Steps in recombinant DNA technology:-

The following steps are involved in recombinant DNA technology:-

  • Isolation of genomic DNA from the donor.
  • Fragmentation of the isolated DNA fragment by using restriction enzymes.
  • Screening of the fragments for the desired gene.
  • Insertion of the fragments with the desired gene into a cloning vector so as to develop a recombinant or chimeric DNA.
  • Introduction of the recombinant vector into a competent host cell.
  • Culturing of the cells containing recombinant vectors to obtain multiple copies of the desired fragment of DNA.
  • Use of these copies to transform suitable host cells so as to express the desired gene.
Concept: Plasmids
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