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Solution - Tools of Recombinant Dna Technology - Cloning Vectors

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Question

State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.

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(a) Name the selectable markers in the cloning vector pBR322? Mention the role they play.

(b) Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?

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Draw a schematic sketch of pBR 322 plasmid and label the following in it: (3)

(a) Any two restriction sites.

(b) Ori and rop genes.

(c) An antibiotic resistant gene.

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Solution for concept: Tools of Recombinant Dna Technology - Cloning Vectors. For the courses 12th CBSE (Arts), 12th CBSE (Commerce), 12th CBSE (Science)
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