HSC Science (General) 12th Board ExamMaharashtra State Board

View all notifications

Describe the Methodology of Tissue Culture. - HSC Science (General) 12th Board Exam - Biology

Create free account

      Forgot password?


Describe the methodology of tissue culture.


Methodology of tissue culture:-

1. Explant culture:

  • Explants such as parenchyma tissues or meristems are excised from the plant, sterilised and placed on a solid nutrient medium.
  • The cells from the explants absorb nutrients and begin to multiply.

2. Callus formation and its culture:

  • The proliferation of cells from the explants because of mitosis forms an unorganised mass of cells called callus.
  • All the cells of the callus are identical.

3. Organogenesis:

  • The addition of growth hormones in proper proportion induces the formation of organs.
  • If auxins are more, roots are formed.
  • If cytokinins are more, a shoot system is developed.

4. Formation of cells or suspension culture:

  • The callus is transferred to a liquid nutrient medium and agitated constantly at 100 to 250 rpm.
  • The agitation separates the cells, aerates the mixture and prevents aggregation of cells.
  • Callus and suspension cultures help to achieve cell biomass production which can be used for biochemical isolation, regeneration of new plantlets, formation of transgenic plants and protoplast culture.
  Is there an error in this question or solution?


 2014-2015 (March) (with solutions)
Question 3.1.2 | 3.00 marks

Video TutorialsVIEW ALL [1]

Solution Describe the Methodology of Tissue Culture. Concept: Tissue Culture.
View in app×