Question

The numerical aperture of objective of a microscope is 0.12. The limit of resolution, when light of wavelength 6000A is used to view an object is.......................

1. 0.25 * 10^-7m
2. 2.5 * 10^-7m
3. 25 * 10^-7m
4. 250 * 10^-7m

 

Answer 1

(c) 25 * 10^-7m

The minimum resolvable linear distance between two nearby objects is given by

`"x"=lambda/(2"NA")=(6000xx10^-10)/(2xx0.12)=2.5xx10^-6"m"=25xx10^-7"m"`