The Biological Scissors of Dna Are - Biology


_______ enzymes are used as biological scissors in r-DNA technology.

(A) Restriction endonucleases

(B) DNA ligases

(C) DNA polymerases

(D) Reverse transcriptases



(A) Restriction endonucleases

Concept: DNA Replication
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2014-2015 (October)


How CO2 makes idlies puffy?

As the base sequence present on one strand of DNA decides the base sequence of other strands; this strand is considered as ______.

Very Short Answer Question:

Which enzyme does remove supercoils from replicating DNA?

Very Short Answer Question:

Why are Okazaki fragments formed on lagging strand only?

Very Short Answer Question:

When does DNA replication takes place?

Enlist the names of enzymes used in semiconservative replication of DNA?

Define Heterochromatin.

Draw neat and labelled diagram of Replication Fork.

Okasaki fragments are joined together by ______.

What are Okazaki fragments?

E. coli cell grown on 15N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?

What is the basis for the difference in the synthesis of the leading and lagging strand of DNA molecules?

Which of the following statements about DNA replication is not correct?

Which of the following statements is not true about DNA replication in eukaryotes?

Meselson and Stahl’s experiment proved ____________.

Differentiate - Leading stand and lagging strand.

From their examination of the structure of DNA, What did Watson and Crick infer about the probable mechanism of DNA replication, coding capability, and mutation?

a) Identify the figure given below

b) Redraw the structure as a replicating fork and label the parts

c) Write the source of energy for this replication and name the enzyme involved in this process.

d) Mention the differences in the synthesis of protein, based on the polarity of the two template strands.

Identify the direction of DNA replication in eukaryotes.

Meselson and Stahl used ____________ in the experiment to prove semiconservative DNA replication.

For which of the following purpose agarose gel electrophoresis technique is used?

During DNA replication, Okazaki fragments are used to elongate ____________.

When DNA directs the synthesis of chemical molecules other than itself, then such functions of DNA are known as ______.

At the point of origin, enzyme endonuclease nicks one of the strands of DNA by breaking the ______

In DNA molecule, pairing between two complementary nucleotides takes place by ________ bonds.

Select the mis-matched pair.

Nucleolus is a major center for:

"DNA is considered as genetic material" - Why?

Extension of the plasma membrane in a prokaryotic cell is ______.

Which of the following statement is incorrect?

Wobble position means:

Match the following column.

Column - I Column - II
(A) DNA structure 1. Muller and stadder
(B) Semiconservative replication of DNA 2. Beadle and partum
(C) One gene-one enzyme theory 3. Watson and crick
(D) Induction of mutation 4. Massillon and stahl

Okazaki is known for his contribution to the under tanding of:

During replication of DNA Okazaki fragments are formed in the direction:

Identify the biomolecule that is capable of self-replication.

Okazaki segments are formed during ______.

Which of the following reaction is required for proofreading during DNA replication by DNA polymerase III?

What background information did Watson and Crick had available with them for developing a model of DNA? What was their own contribution?

The nucleic acid synthesis takes place in ______.

In the experiment of Meselson and Stahl, the heavy DNA was separated from light DNA by centrifugation in ______.

In prokaryotes, the primers of lagging strands are removed by ______.

The sequence of nitrogenous bases on DNA molecule is ATCGA. Which of the following is the correct complementary sequence of nitrogenous bases on mRNA molecule?


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