Name the Technology that Has Helped Scientists to Propagate on a Large Scale the Desired Crops in a Short Duration. List the Steps Carried Out to Propagate the Crops by the Said Technique. - Biology


Name the technology that has helped scientists to propagate on a large scale the desired crops in a short duration. List the steps carried out to propagate the crops by the said technique.



Tissue culture is the technique that has helped scientists propagate the desired crops on a large scale in a short duration.

The following steps are carried out to propagate crops by tissue culture:

  • Tissue culture is the process of developing a complete plant from a part of a plant. The plant part is called an explant.

  • Explants can be grown in sterile conditions in special nutrient media to regenerate complete plants.

  • Nutrient media contain a carbon source (such as sucrose), organic salts, vitamins, amino acids and phytohormones.

  • Hence, propagation is achieved for a large number of plants in a short duration. This process is called micropropagation.

  • All the plants obtained by tissue culture are called somaclones since they are genetically identical to each other as well as the parent plant.


Concept: Tissue Culture
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2013-2014 (March) Delhi Set 1

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What is 'tissue culture'?

Describe the methodology of tissue culture.

How are somatic hybrids obtained?

Find out what the various components of the medium used for propagation of explants in vitro are?

Explain micropropagation and somatic hybridization.

Mention two advantages of micro propagation.

Mention one significant difference between Parenchyma and Sclerenchyma.

Mention one significant difference between Symplastic movement and apoplastic movement

In the following questions/statements has four suggested answers. Rewrite the correct answer

Roots and shoots lengthen through the activity of:

(A) Apical meristem

(B) Vascular Cambium

(C) Lateral meristem

(D) Cork Cambium

In the following questions/statements has four suggested answers. Rewrite the correct answer

Opening and closing of stomata is due to:

(A) Ca2+

(B) Na+

(C) K+

(D) Cl–

Give three anatomical differences between a monocot root and a dicot root

Explain the transpiration pull theory for an ascent of sap

Describe the structure of phloem.

Describe different steps involved in tissue culture technique

Give one significant contribution of the given scientists:

P. Maheshwari

Describe the tissue culture technique in plants

How can healthy potato plants be obtained from a desired potato variety which is viral infected? Explain.



Define the term tissue.

Attempt any TWO of the following:

Enlist the applications of tissue culture.

Give one significant difference between :


Answer the following question.
Explain the relevance of "Totipotency" and "Somaclones" in raising healthy banana plants from virus-infected banana plants.

In plant tissue culture auxin is used for ____________.

From the following statements which is NOT correct?

Identify labels P, Q, R in the given figure of root showing different regions.

The large number of genetically identical offsprings produced by micropropogation are called ____________.

To get a disease-free plant from a virus infected plant, which one of the following can be used as an explant in tissue culture?

Which of the following helps bamboo and grasses to elongate?

Removal of ring wood of tissue outside the vascular cambium from the tree trunk kills it because ______.

Which of the following method is NOT used for crop improvement?

Callus is ______. 

Method of production of thousand of plant through tissue culture is ______.

Protoplast is ______.

Why are plants obtained by protoplast culture called somatic hybrids?

What is protoplast fusion?

How is a somatic hybrid different from a hybrid?

It is easier to culture plant cells in vitro as compared to animal cells. Why?

Plants raised through tissue cultures are clones of the ‘parent’ plant. Discuss the utility of these plants.

The pH of nutrient medium for plant tissue culture is in the range of ______.

Explain the concept of tissue culture.

Write a short note on callus culture.

Write a short note on suspension culture.

Give importance of coconut milk in tissue culture.

What does the plant tissue culture medium consists of?


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