Following the collision of two trains a large number of passengers are killed. A majority of them are beyond recognition. Authorities want to hand over the dead to their relatives. - Biology

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Following the collision of two trains a large number of passengers are killed. A majority of them are beyond recognition. Authorities want to hand over the dead to their relatives. Name a modern scientific method and write the procedure that would help in the identification of kinship.

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Solution

DNA fingerprinting is the modern scientific method used for the identification of kinship.

Procedure of DNA Fingerprinting

  • Variable Number of Tandem Repeats (VNTR) are satellite DNAs that show high degree of polymorphism.​ They are used as probes in DNA fingerprinting.

  • Firstly, DNA from an individual is isolated and cut with restriction endonucleases.

  • Fragments are separated according to their size and molecular weight through gel electrophoresis.

  • Fragments separated through electrophoresis gel are blotted (immobilised) on a synthetic membrane such as nylon or nitrocellulose.

  • Immobilised fragments are hybridised with a VNTR probe.

  • Hybridised DNA fragments can be detected by autoradiography.

  • VNTRs vary in size, ranging from 0.1 to 20 kb. Hence, in the autoradiogram, a band of different sizes will be obtained.

  • These bands are characteristics of an individual. They are different in each individual, except in the case of identical twins.

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2014-2015 (March) Delhi Set 1

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RELATED QUESTIONS

Write down the full form of R.F.L.P.


Give the applications of DNA fingerprinting technique.


The genetic marker used as key factor in DNA fingerprinting is _______.

(A) VNTRs

(B) Exons

(C) Introns

(D) DNA probes


Explain the significance of satellite DNA in DNA fingerprinting technique.


“A very small sample of tissue or even a drop of blood can help determine paternity”. Provide a scientific explanation to substantiate the statement.


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In DNA fingerprinting technique, radioactive DNA probe is obtained from______ of female banded krait snake.

(a) X chromosome
(b) Y chromoson1e
(c) X and Y chromosomes
(d) autosome


Name the radioactive probe used in India for DNA fingerprinting.


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Briefly describe the technique employed in DNA fingerprinting.


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How is repetitive/satellite DNA separated from bulk genomic DNA for various genetic experiments?

 


"A very small sample of tissue or even a drop of blood can help determine paternity." Provide a scientific explanation to substantiate how it is possible.


Short Answer Question:

Match the entries in column I with those of column II and choose the correct answer.

Column I Column II
A. Alkali treatment i. Separation of DNA fragments on gel slab
B. Southern blotting ii. Split DNA fragments into single strands
C. Electrophoresis iii. DNA transferred to nitrocellulose sheet
D. PCR iv. X-ray photography
E. Autoradiography v. Produce fragments of different sizes
F. DNA treated with REN vi. DNA amplification

What are the different types of mutations?


Explain different steps involved in DNA Fingerprinting.


rDNA is a ______.


DNA fingerprinting is based on the principle of identifying______.


Restriction endonucleases cut the DNA molecule at specific positions known as ______.


Similar DNA fingerprinting is obtained for______.


In gene cloning the DNA of interest is integrated in a ______.


DNA fingerprinting technique was developed by Alec Jeffrey


Assertion: rDNA is superior over hybridisation techniques.

Reason: Desired genes are inserted without introducing the undesriable genes in target organisms.


State the applications of DNA fingerprinting technique.


Which of the following are the applications of DNA fingerprinting?


Which of the following technique is used for amplification or multiplication of DNA for fingerprinting?


Identify the CORRECT description of DNA fingerprinting.


____________ are the DNA sequences which are useful in DNA fingerprinting.


ln which of the following technique DNA bands are transferred from agarose gel to nitrocellulose or nylon membrane?


In DNA fingerprinting technique, the radioactive intermediates formed during hybridization are______ molecules.


Identify the criterion for movement of DNA fragments on agarose gel during gel electrophoresis.


The process in which sample DNA and probe DNA form double stranded DNA is called ____________.


Variable number of tandem repeats has significance in ______.


The DNA fingerprinting pattern of the child is ______ 


Indole-3-acetic acid (auxin) was first isolated from ______.


Polymorphism arises due to ______


Which of the following technique is used m DNA fingerprinting?


DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as ______


Would it be appropriate to use DNA probes such as VNTR in DNA finger printing of a bacteriaphage?


List the various markers that are used in DNA finger printing.


The technique of DNA fingerprinting was first given by ______.


Name and describe the steps involved in the technique widely used in forensics that serves as the basis of paternity testing in case of disputes.


Match the following pairs:

  Column I   Column II
(i) PCR (a) DNA transferred to nylon membrane
(ii) Electrophoresis (b) DNA amplification
(iii) Southern blotting (c) Restriction digestion
(iv)  Autoradiography (d) Produce fragments of different size
(v) Alkali treatment (e) Separation of DNA fragments on gel slab
(vi) DNA treated with REN (f) X-ray photography

Arrange the following steps of DNA fingerprinting in correct sequence:

  1. Gel electrophoresis
  2. Isolation of DNA
  3. Southern blotting
  4. Restriction digestion

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