Find out a qualitative test for proteins, fats and oils, amino acids and test, any fruit juice, saliva, and urine for them.
Biuret test for protein : The biuret test is a chemical test used for determining the presence of peptide bonds. In a positive test, a copper II ion (Cu2+ ion) is reduced to copper I (Cu+) which forms a complex with the nitrogen and carbon of peptide bonds in an alkaline solution. A violet colour indicates the presence of proteins.
Ninhydrin test for amino acid: Ninhydrin (2,2 Dihydroxy indane-l,3-dione) is a chemical used to detect ammonia or primary and secondary amines. When reacting with these free amines, a deep blue or purple colour known as Ruhemann’s purple is evolved. Amino acid analysis of proteins is also done by ninhydrine. Most of the amino acids (including a-amino acids) are hydrolysed and reacted with ninhydrin except proline (a secondary amine). Amino acid containing a free amino group and a free carboxylic acid group reacts together with ninhydrin to produce coloured product. When the amino group is secondary, the condensation product is yellow.
Solubility test for fats and oils : A positive solubility test for fats is that the fat dissolves in lighter fluid and not in water. In this test, 5 drops of fat or oil are added in two test tubes containing 10 drops of lighter fluid and 10 drops cold water respectively
Fruit juice contains sugar so it cannot be tested by the above mentioned tests. Saliva contains proteins, mineral salts, amylase etc., so it can be tested for protein and amino acids. Urine contains proteins, so it can be tested for it.
Video Tutorials For All Subjects
- Introduction of Biomolecules