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Explain Briefly Restriction Enzymes and Dna - Biology

Explain briefly Restriction enzymes and DNA

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Restriction enzymes are molecular scissors used in molecular biology for cutting DNA sequences from a specific site. It plays an important role in gene manipulation. The enzymes recognize a specific six-box pair sequence known as the recognition sequence and cut the sequence at a specific site. For example, the recognition site for enzyme ECORI is as follows:

Restriction enzyme are categorized into two types −

(i) Exonuclease − It is a type of restriction enzyme that removes the nucleotide from either 5 or 3’ ends of the DNA molecule.

(ii) Endonuclease − It is a type of restriction enzyme that makes a cut within the DNA at a specific site. This enzyme acts as an important tool in genetic engineering. It is commonly used to make a cut in the sequence to obtain DNA fragments with sticky ends, which are later joined by enzyme DNA ligase

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NCERT Class 12 Biology Textbook
Chapter 11 Biotechnology : Principles and Processes
Q 11.2 | Page 206
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