Advertisement Remove all ads

Explain Briefly Restriction Enzymes and Dna - Biology

Advertisement Remove all ads
Advertisement Remove all ads
Advertisement Remove all ads

Explain briefly Restriction enzymes and DNA

Advertisement Remove all ads


Restriction enzymes are molecular scissors used in molecular biology for cutting DNA sequences from a specific site. It plays an important role in gene manipulation. The enzymes recognize a specific six-box pair sequence known as the recognition sequence and cut the sequence at a specific site. For example, the recognition site for enzyme ECORI is as follows:

Restriction enzyme are categorized into two types −

(i) Exonuclease − It is a type of restriction enzyme that removes the nucleotide from either 5 or 3’ ends of the DNA molecule.

(ii) Endonuclease − It is a type of restriction enzyme that makes a cut within the DNA at a specific site. This enzyme acts as an important tool in genetic engineering. It is commonly used to make a cut in the sequence to obtain DNA fragments with sticky ends, which are later joined by enzyme DNA ligase

Concept: Tools of Recombinant DNA Technology - Restriction Enzymes
  Is there an error in this question or solution?
Advertisement Remove all ads


NCERT Class 12 Biology
Chapter 11 Biotechnology : Principles and Processes
Exercises | Q 11.2 | Page 206
Advertisement Remove all ads

View all notifications

      Forgot password?
View in app×