- Origin of replication (ori), Selectable marker, Cloning sites, recognition sites,antibiotic resistance, Vectors for cloning genes in plants and animals
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.